TestRX testosterone booster review

TestRX is a testosterone booster to help boost all your manly goals.

More specifically, it’s said to help to increase muscle mass, increase energy, boost strength, help with fat loss and crank up your sex drive.

Will it perform or not?

Let’s analyze each ingredient. If you want a quick answer, scroll to the summary below.

TestRX ingredients

Ingredients discussed

Vitamin D

Low vitamin D has been associated with low testosterone and that’s why most testosterone boosters contain vitamin D.

Vitamin D is positively associated with total testosterone (TT), free testosterone levels (fT) and lower SHBG (RRR).

This one study showed that supplementing 3 332 IU vitamin D for 1 year saw a 2.7nmol/L (78ng/dl) increase in testosterone (R). If someone had a testosterone of 400ng/dl, it would increase to 478ng/dl after 1 year.

However, most other studies show that vitamin D supplementation doesn’t increase testosterone. 💊❌♂

  • 20,000IU per week for 12 weeks: “We found no significant treatment effect on serum TT” (R)
  • 600 to 1200IU daily for 16 weeks: “did not affect mean testosterone concentrations at the end of the study” (R)
  • 800IE daily for 12 weeks: “Testosterone levels did not change significantly” (R)

Although vitamin D supplementation doesn’t increase your testosterone, sunlight does!

💊❌♂ ➡️ ☀️✅♂

But it’s not that vitamin D is useless. It obviously has a lot of benefits.

A few benefits include:

  • Inhibiting the deactivation of androgens from the androgen receptors by inhibiting the glucuronidation (a mechanism that inactivates and eliminates substances) of DHT, resulting in an accumulation of DHT to exert a more powerful effect. (R)
  • Increasing androgen receptors as well as the androgen affinity to their receptors. (RR)
  • Increasing IGF-1 (R). IGF-1 stimulates 5-alpha reductase leading to more DHT.

But alas, as this 2020 study writes it quite elegantly: “Mechanistically, vitamin D exhibits essential roles in the testis and prostate; otherwise, there is no apparent evidence to support the use of vitamin D supplementation to increase testosterone levels and to improve clinical outcomes related to the male reproductive system.

If I were to make a testosterone booster, I would not add vitamin D to it. Getting lots of sunlight will increase testosterone and have a much better impact on your health than vitamin D supplementation.

☀️Why every male should get sunlight daily☀️

Vitamin K2

Vitamin K2 on testosterone

Vitamin K2 MK4 preferentially accumulates in the testes vs the liver (189.5 vs 0.634). Supplementing MK-4 massively increases MK-4 in the testes as well (from 189.5 to 995.9).

Vitamin K2 deficiency massively decreases testosterone without affecting LH (R).

In the testes, MK-4 supplementation enhances testicular sensitivity to LH and upregulates various steroidogenic enzymes to boost testosterone production.

In summary, MK-4 increased testosterone by an average of 56% and even up to 70% in some rats. MK-4 increased intratesticular testosterone by 88%.

Two main enzymes that are upregulated by MK-4 are StAR and CYP11A1.

  • StAR; the rate-limited enzyme that transports cholesterol into the mitochondria to be converted to pregnenolone.
  • CYP11A1; the enzyme that converts cholesterol into pregnenolone.

The dose used in the study above was 75mg/kg, which translates to 12.16mg/kg for humans, which is a really high dose; 1216mg for a 100kg guy.

A novel way to get high amounts of vitamin K2 MK-4 into the nutsack is to apply MK-4 directly on the testes instead of taking it orally.

TestRX only contains 20mcg vitamin k2 (likely MK-7), so that’s unlikely enough to have a testosterone-boosting effect.

Vitamin K2 on estrogen

Vitamin K2 activates the enzyme 17β-HSD type 4; which converts 17β-estradiol into estrone (R). Estradiol is our most potent estrogen, whereas estrone is relatively weak. By reducing the estradiol to estrone ratio, vitamin K2 can help against hyperestrogenism.

Vitamin K2 on DHEA

And that’s not all, type 4 17 beta-HSD also converts androstenediol into dehydroepiandrosterone (DHEA). DHEA can then be converted to androstenedione (precursor to testosterone) to androsterone (precursor to DHT).

Vitamin K2 decreases estradiol (the most powerful estrogen) as well as increases DHEA at the same time.

The best food source of vitamin K2 (MK-4) is bone marrow, followed by goose pate.

Vitamin B6

Most supplements add vitamin B6 because it helps with magnesium retention, but it actually has many other benefits as well.

When vitamin B6 is low, this can lead to:

  • Low testosterone, despite normal LH (R)
  • High prolactin (R)
  • High adrenaline (R)
  • Enhanced estrogen sensitivity
  • Enhanced cortisol sensitivity (R)
  • Low dopamine (R)
  • And a lot more negative things.

TestRX contains only 5mg of vitamin B6, which is a good amount to prevent overload and toxicity.

Other good vitamin B6 sources are meat, organ meat, bananas, avocado, etc.

D-Aspartic Acid

D-aspartic acid on LH

In humans and rats, sodium D-aspartate induces an enhancement of LH release.

In the rat pituitary, sodium D-aspartate increases the release and synthesis of LH through the involvement of cGMP as a second messenger (R). cGMP is broken down by phosphodiesterase enzymes (PDEs).

PDE 5, -6 and -9 are cGMP-specific while PDE1, -2, -3, -10 and -11 can break down both cAMP and cGMP. PDE1, PDE2, PDE4, and PDE11A are highly expressed in the pituitary. Thus a PDE1, 2 and 11 inhibitor together with aspartic acid might enhance the LH release even more.

In this graph, you can see how D-aspartic acid promotes the release and cGMP in a dose-dependent manner. As cGMP goes up, so does LH.

In humans, 3g Aspartic acid doubled LH after 4 weeks (R). However, the increase in total testosterone was only 80ng/dl. Which isn’t bad, since these guys already had high testosterone.

does D-aspartic acid increase testosterone?

🧨How to increase LH and optimize your testosterone to LH ratio

D-aspartic acid on testosterone

Since aspartic acid accumulates in the testes, you’d assume it’s important for testosterone optimization right? Correct!

Depleting aspartic acid tanks testosterone levels.

A few of the mechanisms include lower cAMP levels and reduced StAR and 17β-HSD expression (RRR).

  1. cAMP enhances testicular sensitivity to LH.
  2. StAR transports cholesterol into the mitochondrial of the Leydig cells to be converted to pregnenolone.
  3. 17β-HSD converts androstenedione into testosterone.

The average increase in testosterone in the 3g group was 72ng/dl. Not too bad.

TestRX contains 2.3g D-aspartic acid, which is slightly below the desired 3g dose.

🥛 Find out which foods contain the most D-aspartic acid (you’ll be surprised)


There are 6 studies showing that Fenugreek can increase testosterone.

Compiling all of the studies, the average increase in testosterone was 67ng/dl. Some got as low as a 31ng/dl increase and others a 124ng/dl increase on average.

Also, some people are more likely to be responders and non-responders. Someone might get a 0 increase whereas another might get a 500ng/dl increase from the same supplement.

TestRX contains a 50% saponin extract, which might or might not be able to increase testosterone (it hasn’t been studied).

However, most Fenugreek extracts (regardless of the extract) increase testosterone, sexual function and exercise performance. For the best effects, you want to use 500-600mg of Fenugreek extract daily.

TestRX contains only 300mg, which might be insufficient since most other studies use 500-600mg per day.

🧨Fenugreek on testosterone, dopamine and being Alpha


Zinc is one of the most important minerals used for testosterone optimization. This is because it can have a powerful effect on boosting testosterone. However, it will only increase testosterone if you’re deficient in zinc.

Low zinc in the body leads to:

  • Low testosterone
  • High estrogen
  • Very low testosterone-to-estrogen ratio
  • Low DHT
  • High 3alpha-diol (weak DHT metabolite) and
  • An awful DHT to 3 alpha-diol ratio.

A low meat (low zinc) diet can tank your testosterone.

An interesting study was done where they put 22 men on a zinc-deficient diet for 24 weeks to see what would happen to their testosterone (R).

Their diet before the experimental diet was roughly 12.5mg, which is just above the recommended daily allowance (RDA). The zinc content of the experimental zinc-deficient diet was 4.2-5.6mg. 2-3 times less of what they usually consumed.

The low-zinc foods were from a semipurified diet based on texturized soy products with added vitamins and minerals, except zinc. I can’t believe anyone ate that for 20 weeks!

As a result, their testosterone dropped from 1150ng/dl to 305nd/dl after 20 weeks! That’s roughly an x4 drop.

As a side note, it’s quite remarkable that their baseline testosterone was 1150ng/dl since they worked in a hospital, which is demanding, can be stressful and has a severe lack of sunlight.

Supplementing zinc can double your testosterone

The same researchers did another study where that supplemented men between the ages of 50 and 80 with 30mg zinc gluconate daily for 6 months (R).

Before the supplemental protocol, their average daily dietary zinc intake was a measly 69% of the RDA. Since the RDA for zinc is 11mg, that would mean they were consuming only 7.6mg of zinc daily.

Over the 6 months, their testosterone doubled from 240ng/dl to 460ng/dl, putting them in the normal range (R).

Another study found that supplementing 240mg zinc daily for 40-50 days increased testosterone by nearly 200ng/dl. And as you can see, DHT also nearly doubled.

Zinc increases testosterone mainly by:

  • Being a cofactor for the enzyme, 17 β-hydroxysteroid dehydrogenase (17β-HSD), which converts androstenedione to testosterone (R).
  • Inhibiting aromatase
  • Lowering excess cortisol
  • Lowering prolactin
  • Increasing dopamine
  • Improving thyroid function
  • Lowering inflammation
  • Improving insulin sensitivity
  • Increases undercarboxylated osteocalcin, which is the hormonally active form, stimulate testosterone synthesis (A).
  • etc.

👉 Zinc on testosterone, DHT and being Alpha

Magnesium - Free education icons


Supplemental magnesium can increase testosterone, which is shown by multiple research papers (RR).

Magnesium supplementation is able to increase testosterone in sedentary, beginner and advanced athletes and it potentiates the testosterone boost induced by exercise (R). The dose used in this study was 10mg/kg of magnesium sulfate (Epsom salt), which has roughly 1mg of elemental magnesium (10%) per 10mg.

ZMA (30mg zinc, 450mg magnesium and 10mg B6) was able to dramatically increase testosterone (184ng/dl) in football players over an 8-week period. Very good boost if you ask me since the placebo group experienced a decline (R). It’s unlikely that zinc alone was responsible for this since zinc hasn’t been shown to cause such big boosts.

Here are 6 ways how magnesium can increase testosterone:

  • Lowering gut inflammation and preventing leaky gut
    • A diet low in magnesium leads to a decrease in bifidobacteria in the gut as well as an increase in gut permeability (R). This allows toxins to enter the body, causing inflammation and lower testosterone.
  • Increase ATP production, which promotes the production of testosterone in a dose-dependent manner (RR).
    • Magnesium increases ATP production by directly stimulating ATP synthase (the enzyme that creates ATP) (R).
    • Binding to ATP to stabilize it.
  • Improve insulin sensitivity
    • At least 25% of men with type 2 diabetes have low testosterone (R).
  • Lower excess cortisol
  • Improve thyroid hormone production
  • Activating vitamin D
    • Vitamin D can upregulate various steroidogenic enzymes
  • Activation of vitamin B1.

👉 Magnesium for boosting testosterone and being Alpha


I have 2 main problems with this supplement.

  1. Fenugreek is underdosed
  2. D-aspartic acid is best cycled

Although the ingredients aren’t bad, this product will not provide the strongest punch. Plus, having to cycle D-aspartic acid 2 weeks on and 1 week off, might take away even more from the effectiveness of the other ingredients.

Will TestRX help build strength, increase energy and help with fat loss?

It contains the right ingredients that will help with this. But when it comes to building muscle and losing fat, the effects of supplements draft that of eating right and living a good lifestyle.

Will you feel something while using TestRX?

Most likely yes. The ingredients have been shown to improve mood and sexual function.

Will TestRX increase your testosterone?

Maybe by 100ng/dl on average. Testosterone boosters don’t negate the need for eating better and living a good lifestyle.

📖Related articles to help you become more of an Alpha Energy Male

>1000ng/dl Testosterone: My Step-by-Step Guide on How I Do It Naturally!

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